SpCas9-NG binary vector with a modified sgRNA scaffold starting with a GCCCC sequence
Publication
Qin R, Li J, Liu X, Xu R, Yang J, Wei P. \
Plasmid Copy
High Copy
Bacterial Resistance
Spectinomycin and Kanamycin
Growth Strain
XL1 Blue
Growth Temperature
37°C
Plasmid Size (bp)
17097
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This plasmid contains part of 2019-nCoV(SARS-CoV-2) RdRP gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.