Cat. No.: MC_0000011
   Sequence by GenScript        Sequence by Depositor


Name Cat. No. Availability Price
pCas MC_0000011 4 µg of lyophilized plasmid $65 Edit in GenSmart Design
This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
  • General
  • Specification
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  • Keywords CRISPR
    Vector Backbone pKD46
    Source Member Deposit
    Depositor Sheng Yang
    Organization Shanghai Institutes for Biological Sciences, CAS
    Description Constitutive expression of Cas9 together with inducible expression of lambda RED and sgR
    Publication Y. Jiang, B. Chen, C. Duan, B. Sun, J. Yang, and S. Yang, \'Multigene Editing in the Escherichia Coli Genome Via the Crispr-Cas9 System\', Appl Environ Microbiol, 81 (2015), 2506-14.
    Plasmid Copy Low Copy
    Bacterial Resistance Kanamycin
    Growth Strain DH5-Alpha
    Growth Temperature 30°C
    Plasmid Size (bp) 12542
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