ClyA RR plasmid from Shuo Huang

Sequence by GenScript Sequence by Depositor

ClyA RR

Cat. No.:

MC_0068760

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$65

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General

Specification

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Keywords	Other
Vector Backbone	pET-30a(+)
Source	Member Deposit
Depositor	Shuo Huang
Organization	Nanjing University
Description	The plasmid ClyA-RR is designed for the preparation of a mutant Cytolysin A (ClyA). ClyA is a pore-forming toxin which was found in various E. coli strains. Its structure has been characterized using X-ray crystallography (PDB: 2WCD).With a unique wide aperture, ClyA along with its mutants have been used as nanopore sensors for the detection of macromolecules. The ClyA-RR mutant is engineered for more efficient DNA sensing (Franceschini L, Brouns T, Willems K. et al. DNA Translocation through Nanopores at Physiological Ionic Strengths Requires Precise Nanoscale Engineering. ACS Nano 2016, 10(9): 8394-8402). It is also a highly compatible sensor for the imaging based DiffusiOptoPhysiology platform (Wang Y, Wang Y, Du X, et al. Electrode-free nanopore sensing by DiffusiOptoPhysiology[J]. Science Advances, 2019, 5(9): eaar3309.)
Publication	Wang Y, Wang Y, Du X, et al. Electrode-free nanopore sensing by DiffusiOptoPhysiology[J]. Science Advances, 2019, 5(9): eaar3309

Plasmid Copy	Low Copy
Bacterial Resistance	Kanamycin
Growth Strain	TOP10
Growth Temperature	37°C
Plasmid Size (bp)	6183
Gene/Insert Name	(ClyA RR)_PET-30a(+)
Gene/Insert Sequence	CATATGACAGGAATATTTGCAGAACAAACTGTAGAAGTAGTTAAATCAGCTATTGAGACCGCGGATGGTGCGCTGGACCTGTATAACAAGTACCTGGACCAGGTGATCCCGTGGAAGACCTTCGATGAAACCATTAAGGAACTGAGCCGTTTTAAACAGGAGTATAGCCAAGAAGCGAGCGTGCTGGTTGGTCGTATCAAGGTGCTGCTGATGGACAGCCAGGATAAATACTTCGAGGCGACCCAAACCGTTTATGAATGGGCGGGTGTGGTTACCCAGCTGCTGAGCGCGTACATTCAACTGTTTGACGGCTATAACGAGAAGAAAGCGCGTGCGCAGAAAGATATCCTGATTCGTATCCTGGACGATGGTGTTAAGAAACTGAACGAAGCGCAGAAGAGCCTGCTGACCAGCAGCCAAAGCTTCAACAACGCGAGCGGCAAACTGCTGGCGCTGGACAGCCAACTGACCAACGATTTTAGCGAGAAGAGCAGCTACTATCAGAGCCAAGTGGACCGTATCCGTAAAGAAGCGTACGCGGGTGCGGCGGCGGGTATTGTTGCGGGTCCGTTCGGCCTGATCATTAGCTATAGCATCGCGGCGGGTGTGGTTGAGGGCAAGCTGATTCCGGAACTGAACAACCGTCTGAAAACCGTGCAGAACTTCTTTACCAGCCTGAGCGCGACCGTTAAGCAAGCGAACAAAGACATCGATGCGGCGAAGCTGAAACTGGCGACCGAGATTGCGGCGATCGGCGAAATTAAGACCGAAACCGAAACCACCCGTTTTTACGTGGATTATGACGATCTGATGCTGAGCCTGCTGAAGGGTGCGGCGAAGAAAATGATCAACACCAGCAACGAGTACCAGCAACGTCACGGCCGTAAGACCCTGTTTGAGGTTCCGGACGTGGGTAGCAGCTATCATCATCATCATCATCATTAATGACTCGAG

*This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.

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