Yaokang Wu(武耀康)
Lab Information
Lab of Food Synthetic Biology and Bio-manufacture (FSBB)-食品合成生物学与生物制造团队
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Metabolic Engineering 0 Synthetic Biology 0 CRISPR / Cas9 0 CRISPR/Cpf1 0 Bacillus subtilis 1
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Wu, Y., Li, Y., Jin, K., Zhang, L., Li, J., Liu, Y., Du, G., Lv, X., Chen, J., Ledesma-Amaro, R., Liu, L., 2023. CRISPR–dCas12a-mediated genetic circuit cascades for multiplexed pathway optimization. Nat Chem Biol 19, 367–377. https://doi.org/10.1038/s41589-022-01230-0
Wu, Y., Chen, T., Liu, Y., Tian, R., Lv, X., Li, J., Du, G., Chen, J., Ledesma-Amaro, R., Liu, L., 2020. Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtilis. Nucleic Acids Research 48, 996–1009. https://doi.org/10.1093/nar/gkz1123
Wu, Y., Chen, T., Liu, Y., Lv, X., Li, J., Du, G., Ledesma-Amaro, R., Liu, L., 2018. CRISPRi allows optimal temporal control of N-acetylglucosamine bioproduction by a dynamic coordination of glucose and xylose metabolism in Bacillus subtilis. Metab. Eng. 49, 232–241. https://doi.org/10.1016/j.ymben.2018.08.012
Wu, Y., Liu, Y., Lv, X., Li, J., Du, G., Liu, L., 2020. CAMERS‐B: CRISPR/Cpf1 assisted multiple‐genes editing and regulation system for Bacillus subtilis. Biotechnol Bioeng 117, 1817–1825. https://doi.org/10.1002/bit.27322
Wu, Y., Li, Y., Zhang, Y., Liu, Y., Li, J., Du, G., Lv, X., Liu, L., 2023. Efficient Protein Expression and Biosynthetic Gene Cluster Regulation in Bacillus subtilis Driven by a T7-BOOST System. ACS Synth. Biol. 12, 3328–3339. https://doi.org/10.1021/acssynbio.3c00331
Wu, Y., Liu, Y., Lv, X., Li, J., Du, G., Liu, L., 2020. Applications of CRISPR in a Microbial Cell Factory: From Genome Reconstruction to Metabolic Network Reprogramming. ACS Synth. Biol. 9, 2228–2238. https://doi.org/10.1021/acssynbio.0c00349Sun, G., Wu, Y., Huang, Z., Liu, Y., Li, J., Du, G., Lv, X., Liu, L., 2023. Directed evolution of diacetylchitobiose deacetylase via high-throughput droplet sorting with a novel, bacteria-based biosensor. Biosensors and Bioelectronics 219, 114818. https://doi.org/10.1016/j.bios.2022.114818Zhu, X., Wu, Y., Lv, X., Liu, Y., Du, G., Li, J., Liu, L., 2022. Combining CRISPR–Cpf1 and Recombineering Facilitates Fast and Efficient Genome Editing in Escherichia coli. ACS Synth. Biol. 11, 1897–1907. https://doi.org/10.1021/acssynbio.2c00041
Sun, G., Wu, Y., Huang, Z., Liu, Y., Li, J., Du, G., Lv, X., Liu, L., 2023. Directed evolution of diacetylchitobiose deacetylase via high-throughput droplet sorting with a novel, bacteria-based biosensor. Biosensors and Bioelectronics 219, 114818. https://doi.org/10.1016/j.bios.2022.114818
Zhu, X., Wu, Y., Lv, X., Liu, Y., Du, G., Li, J., Liu, L., 2022. Combining CRISPR–Cpf1 and Recombineering Facilitates Fast and Efficient Genome Editing in Escherichia coli. ACS Synth. Biol. 11, 1897–1907. https://doi.org/10.1021/acssynbio.2c00041
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