The gRNA guides the CRIPR Cas9 system to the right genomic location for performing a double strand break.The targerting specificity of the CRISPR Cas9 system is determined by the 20 nucleotides at the 5' end of the gRNA. The gRNA base pairs to the complementary strand of the ...Learn More

Q: What are the free tools for plasmid design and mapping?Some of the free tools for plasmid design and mapping are following:1. GenScript2. Molecular Cloud - Gensmart3. SnapGene4. Benchling5. A Plasmid Editor ...Learn More

Q: How many cloning methods have been developed? . There are two main methods of cloning a DNA fragment, namely Molecular Cloning and PCR (Polymerised Chain Reaction)The fundamental difference between the two methods is that molecular cloning involves replication of the DNA in a ...Learn More

Q: What are the facts that determine plasmid copy number? The copy number refers to the number of molecules of an individual plasmid that are normally found in a single bacterial cell. The factors that control copy number are not well understood. Some plasmids, especially the ...Learn More

Q: What are the components of a plasmid vector?Components of a plasmid vector are: Ori (Origin of replication) :The DNA sequence which allows initiation of replication by recruiting transcriptional machinery proteins.Insert (Multiple Cloning Site): an element on the plasmid ...Learn More