pNICKclos1.0 plasmid from Sheng Yang
Keywords | CRISPR |
Vector Backbone | pIMP1-ptb |
Source | Member Deposit |
Depositor | Sheng Yang |
Organization | Center for Excellence in Molecular Plant Sciences (CEMPS), CAS |
Description | Composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging |
Publication | Q. Li, J. Chen, N. P. Minton, Y. Zhang, Z. Wen, J. Liu, H. Yang, Z. Zeng, X. Ren, J. Yang, Y. Gu, W. Jiang, Y. Jiang, and S. Yang, \\\'Crispr-Based Genome Editing and Expression Control Systems in Clostridium Acetobutylicum and Clostridium Beijerinckii\\\', Biotechnol J, 11 (2016), 961-72. |
Plasmid Copy | Low Copy |
Bacterial Resistance | Ampicillin |
Growth Strain | DH5-Alpha |
Growth Temperature | 37°C |
Plasmid Size (bp) | 11081 |
Gene/Insert Name | Cas9 nickase |