pLCNICK plasmid from Sheng Yang

   Sequence by GenScript        Sequence by Depositor

pLCNICK

Cat. No.:
MC_0000244
Availability:
4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)
Price:
$65
  • General
  • Specification
  • Comments (2)
  • Keywords CRISPR
    Vector Backbone pCas
    Source Member Deposit
    Depositor Sheng Yang
    Organization Center for Excellence in Molecular Plant Sciences (CEMPS), CAS
    Description CRISPR
    Publication X. Song, H. Huang, Z. Xiong, L. Ai, and S. Yang, \\\'Crispr-Cas9(D10a) Nickase-Assisted Genome Editing in Lactobacillus Casei\\\', Appl Environ Microbiol, 83 (2017).
    Plasmid Copy Low Copy
    Bacterial Resistance Kanamycin
    Growth Strain DH5-Alpha
    Growth Temperature 30°C
    Plasmid Size (bp) 14369
    Gene/Insert Name Cas9 nickase
    • Yiyong
      21:46 3/12/2020
      I want to do a crispr research in Lactic acid bacteria, so I want this plasmid.
      Reply(0)
      0
    • 刘树文
      23:40 3/17/2019
      It is a very convenient editing platform in Lactobacillus casei. But it seems like a medium-low copy plasmid for extraction.
      Reply(1)
      2
      • Sheng
        09:30 10/17/2019
        @ Yes, sometimes you need midi-preparation of pLCNICK for reciept cells of low-transformation effieciency.
        Reply
        2
    *This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
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