What is nucleic acid detection and analysis?

Manini Penikalapati 16:00:09 05/28/2020

Nucleic acid detection and analysis is accomplished using recombinant DNA technology to isolate individual genes and characterize them based on their proteins. This technology can be carried out through many different protocols depending on the specifics of the nucleic acid(s) present, including radiolabelling, staining, tagging, probing, and hybridization. Nucleic acid hybridization involves the interaction of single-stranded nucleic acids so that hybrids are formed that have similar sequences. Nucleic acid hybrids can be formed between two strands of DNA, two strands of RNA, or one strand of DNA and one of RNA. Hybridization allows for the detection of sequences that are complementary to the isolated nucleic acid of concern, which is radiolabelled. This radioactive nucleic acid is then used as a probe for hybridization to complementary sequences of DNA/RNA

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