Ternary Vector System

Our current research focuses on the plant gene editing with the ternary vector system. Recently, we developed a novel ternary vector system to integrate the MR (Morphogenic Regulator) and CRISPR/Cas modules. This ternary vector system is composed of new pGreen-like binary vectors including pGreen3 and a pVS1-based virulence helper plasmid, which also functions as a replication helper for the pGreen3 vectors in Agrobacterium tumefaciens.

The plasmids, manual  and tips can be found in my personal page. Leave your comments if you have any questions about our research.