Strategies to Efficiently Generate KO/KI Cell Lines

CRISPR/Cas9 is an easy and efficient tool to study gene function in cells. However, generation of CRISPR-mediated gene knock-in or knock-out cell line involves substantial workload, especially for hard-to-transfect cell lines. A wise design of gRNA/donor template is necessary for generating functional gene KO/KI and improve editing efficiency.