Plant Expression Vector Construction

Creative Biogene is an expert in plant genetic engineering and molecular biology. We have devoted many years of efforts in optimizing plant expression vectors to adapt to different plant species. We provide universal and customized plant expression vector construction services, covering from sequence optimization, gene synthesis/cloning, DNA fragment assembling, sequencing to plasmid preparation. With advanced technologies and highly experienced staffs, we will work closely with you to bring satisfied plant expression vector construction services.

Gene cloning and vector construction are routine procedures in plant genetic engineering. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. The generation of genetically modified crops with valued traits requires different combinations of expression elements to achieve optimum effects. In addition, a right choice of expression construct also plays an important role in research of gene function and promoter activity analysis.

Service

• Overexpression vector construction: Fully customizable vector that you can choose specific promoter like inducible promoter (pCab, pEr), constitutive promoter (p35s, pUbi, pNos) and tissue-specific promoter (pRbcs, pGlu, pGt1).
• Silencing vector construction: RNAi vector for stable gene silencing. VIGS (virus-induced gene silencing) vector for transient gene silencing.
• Gene knockout vector construction: The cas9 gene sequence has been codon optimized to improve the expression in plant. AtU6 promoter drives sgRNA expression in dicots and OsU3/OsU6 drives sgRNA in monocots.
• Promoter activity analysis vector construction: Your target promoter will be fused to GusPlus gene for histochemical GUS staining use.
• Complementary expression vector construction: Native promoter drives gene expression.