How should gRNA sequences be designed?

Designing your gRNA sequences involves 4 steps:

1. Determining the target gene locus.
2. Finding suitable sequences for Cas9 targeting.
3. Checking the potential for off-target binding.
4. Selecting gRNAs sequences that lie within your preferred binding region.

GenScript's gRNA database and online design tool will take out much of the guesswork when you're choosing gRNA sequences, by providing off-target scores and chromosomal location.