Get a Free CRISPR Plasmid for Bacillus subtilis!

MolecularCloud Team 01:34:16 06/02/2020

MolecularCloud plasmid repository includes more than 100,000 plasmids shared by researchers, gRNA plasmids and ORF plasmids from GenScript. Every month, we pick out 3 plasmids from the repository as exclusive benefits for MolecularCloud members.

Free plasmids for June come from Dr. Long Liu’s CRISPR/Cpf1 assisted multiple-genes editing and regulation system for Bacillus subtilis (CAMERS-B). This system can achieve the double genes in-frame knocking out, multiple point mutations (up to six), or single gene insertion at a time with 100% efficiency.

Free Plasmids for June

MC ID	Name	Description
MC_0068418	pHT-XCR6	The plasmid is used for the expression of Cpf1 and NgAgo in B. subtilis, and can replicate in E. coli.
MC_0068419	pcrF11	The plasmid is used for the expression of crRNA in B. subtilis, and can replicate in E. coli.
MC_0068421	pLCg6-dCpf1	The plasmid is used for the integrated expression of dCpf1(D917A) in B. subtilis, and can replicate in E. coli.

More Plasmids of this article

MC ID	Name	Description
MC_0101074	pHT-XCR2	The plasmid is used for the expression of Cpf1 and λ-Red in B. subtilis，and can replicate in E. coli.
MC_0068650	pLCx-dcpf1	The plasmid is used for the integrated expression of dCpf1(D917A) in B. subtilis，and can replicate in E. coli.
MC_0068424	pcra3	The plasmid is used for the integrated expression of crRNA in B. subtilis，and can replicate in E. coli.
MC_0068423	pcra2	The plasmid is used for the integrated expression of crRNA in B. subtilis，and can replicate in E. coli.
MC_0068422	pLCg6-dCpf1-RemA	The plasmid is used for the integrated expression of dCpf-RemA fusion protein in B. subtilis，and can replicate in E. coli.
MC_0068417	pHT-XC	The plasmid is used for the expression of Cpf1 in Bacillus subtilis，and can replicate in E. coli.

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