GenWand™ Double-Stranded DNA Synthesis Service

Double-stranded DNA (dsDNA) has been used for CRISPR homology directed repair (HDR) template for creating gene knock-in with high editing efficiency. GenWand™ dsDNA is developed with covalently closed ends to mitigate no-homologous end joining risk and increase knock-in accuracy. GenScript now offers up to g level closed-end dsDNA ideal for screening, process optimization and scale up.

Why closed-end dsDNA as CRISPR Gene Knock-In HDR Templates?

• ✔ Lower toxicity and higher KI efficiency compare to PCR product
• ✔ Closed-end to mitigate NHEJ events
• ✔ No introduction of unnecessary plasmid sequences
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• ✔ Ideal for people need to KI long sequence
• ✔ Ideal for large scale screening and scale up

Why GenWand™ dsDNA Service