For antibody generation, are there special requirements for the production of antigen protein?

Elizabeth Kacerek 04:05:19 12/28/2021

The antigen (protein) should be soluble in a PBS or a buffer without organic solvents. Small tags (such as 6xHis, HA, FLAG, V5, c-myc) are preferred. For mAb development, the protein antigen shall be >2mg and >75% purity. For diagnostic or functional mAb development, the higher purity (85-90%) is preferred; for pAb development, protein antigen shall be >3mg and >85% purity (additional 5mg is needed for purified pAb).

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