What is molecular cloning?
Molecular cloning refers to the replication and recombination of DNA molecules, which makes many identical copies of a piece of DNA, such as a gene of a genome, enabling the manipulation and study of genes. The workflow of cloning has four steps:
1. DNA is isolated
2. Isolated DNA is inserted into a cloning vector
3. Vectors are propagated in a host
4. Hosts containing the DNA insert are selected
Cloning small fragments of a genome allows specific genes, their protein products, and non-coding regions to be studied in isolation.
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