What are the necessary components of a plasmid? #Every is a writer

What are the necessary components of a plasmid?

The plasmid used for cloning and expression usually including three major components:

1. Replicon: Also known as the replication initiation region, it contains genetic factors that control the origin of plasmid DNA replication and plasmid copy number.

2. Selection marker: The selection marker encoded by the plasmid gives the host cell a new phenotype and is used to identify and screen the host cell transformed with the plasmid.

3. Multiple cloning sites:

Plasmids are small circular DNA molecules. As the most commonly used and simplest vector in genetic engineering.

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