Research Area
DNA Repair, Gene Editing and Carcinogenesis
Team Description
Our group focuses on DNA repair-induced mutagenesis, which underlines the precision of gene editing, the development of certain cancers and aging. We are particularly interested in: 1) Mechanism of DNA repair-induced mutagenesis in gene editing; 2) Development of novel gene editing tools; 3) New proteins and pathways involved in DNA repair-induced mutagenesis; 4) Role of DNA repair-induced mutagenesis in carcinogenesis. We have revealed the mechanism of APOBEC cytidine deaminase-mediated mutagenesis during the repair of CRISPR/Cas9-generated DNA breaks and developed various base editors for different purposes, including enhanced base editors (eBEs) with improved editing precision and efficiency, dCpf1 base editors (dCpf1-BEs) for base editing in A/T-rich regions, hAPOBEC3A base editors (hA3A-BEs) for efficient base editing in G/C rich and/or highly-methylated regions and dCas12a-derived base editors (BEACON) for efficient base editing with only background levels of DNA damage response.
Team Members
Team Show
Publications
1. Yang, Li#*; Chen, Jia#.A Tale of Two Moieties: Rapidly Evolving CRISPR/Cas-Based Genome Editing.TRENDS IN BIOCHEMICAL SCIENCES. 30 Jun 2020. 45(10):874-888.
2. Wang, Xiao*; Ding, Chengfeng; Yu, Wenxia; Wang, Ying; He, Siting; Yang, Bei; Xiong, Yi-Chun; Wei, Jia; Li, Jifang; Liang, Jiayi; Lu, Zongyang; Zhu, Wei; Wu, Jing; Zhou, Zhi; Huang, Xingxu; Liu, Zhen#; Yang, Li#; Chen, Jia#.Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response.CELL REPORTS. 02 Jun 2020. 31(9).
3. Yang, Li#*; Yang, Bei#; Chen, Jia#.One Prime for All Editing.CELL. 12 Dec 2019. 179(7):1448-1450.
4. Wang, Ying*; Gao, Runze; Wu, Jing; Xiong, Yi-Chun; Wei, Jia; Zhang, Sipin; Yang, Bei; Chen, Jia#; Yang, Li#.Comparison of cytosine base editors and development of the BEable-GPS database for targeting pathogenic SNVs.GENOME BIOLOGY. 23 Oct 2019. 20(1).
5. Chen, Jia#*; Yang, Bei#; Yang, Li#.To BE or not to BE, that is the question.NATURE BIOTECHNOLOGY. May 2019. 37(5):520-521.
6. Yang, Bei#*; Yang, Li#; Chen, Jia#.Development and Application of Base Editors.CRISPR JOURNAL. Apr 2019. 2(2):91-104.
7. Li, Jianan*; Liu, Zhen*; Huang, Shisheng*; Wang, Xiao; Li, Guanglei; Xu, Yuting; Yu, Wenxia; Chen, Shanshan; Zhang, Yu; Ma, Hanhui; Ke, Zunfu; Chen, Jia#; Sun, Qiang#; Huang, Xingxu#.Efficient base editing in G/C-rich regions to model androgen insensitivity syndrome.CELL RESEARCH. Feb 2019. 29(2):174-176.
8. Wang, Xiao*; Li, Jianan; Wang, Ying; Yang, Bei; Wei, Jia; Wu, Jing; Wang, Ruixuan; Huang, Xingxu#; Chen, Jia#; Yang, Li#.Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.NATURE BIOTECHNOLOGY. Oct 2018. 36(10):946-+.
9. Pawluk, April*; Chen, Jia#; Ji, Weizhi; Mali, Prashant.The Future of Genome Editing.CELL. 31 May 2018. 173(6):1311-1313.
10. Li, Xiaosa*; Wang, Ying; Liu, Yajing; Yang, Bei; Wang, Xiao; Wei, Jia; Lu, Zongyang; Zhang, Yuxi; Wu, Jing; Huang, Xingxu#; Yang, Li#; Chen, Jia#.Base editing with a Cpf1-cytidine deaminase fusion.NATURE BIOTECHNOLOGY. Apr 2018. 36(4):324-+.
11. Lei, Liqun*; Chen, Hongquan; Xue, Wei; Yang, Bei; Hu, Bian; Wei, Jia; Wang, Lijie; Cui, Yiqiang; Li, Wei; Wang, Jianying; Yan, Lei; Shang, Wanjing; Gao, Jimin; Sha, Jiahao; Zhuang, Min; Huang, Xingxu; Shen, Bin#; Yang, Li#; Chen, Jia#.APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks.NATURE STRUCTURAL & MOLECULAR BIOLOGY. Jan 2018. 25(1):45-+.
12. Wang, Lijie*; Xue, Wei; Yan, Lei; Li, Xiaosa; Wei, Jia; Chen, Miaomiao; Wu, Jing; Yang, Bei#; Yang, Li#; Chen, Jia#.Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor.CELL RESEARCH. Oct 2017. 27(10):1289-1292.
13. Yang, Bei#*; Li, Xiaosa; Lei, Liqun; Chen, Jia#.APOBEC: From mutator to editor.JOURNAL OF GENETICS AND GENOMICS. 20 Sep 2017. 44(9):423-437.